《植物生理学报》 2013, 49(3): 273-276

橡胶树死皮(TPD)发生过程中胶乳蛋白质的泛素化

覃碧^1,*, 刘长仁², 杜磊³

1中国热带农业科学院橡胶研究所农业部橡胶树生物学与遗传资源利用重点实验室, 海南儋州571737; 2海南大学农学院, 海

通信作者：覃碧；E-mail: qinbi126@163.com；Tel: 0898-23301174

摘　要：

以健康和不同级别死皮(TPD)橡胶树为材料, 用Western blot分析橡胶树TPD发生过程中胶乳蛋白质泛素化水平的动态变化, 结果表明, 健康与TPD橡胶树胶乳蛋白质的泛素化水平存在显著差异, 其中2个蛋白的泛素化水平在TPD中显著升高, 而7个蛋白的泛素化水平在TPD中明显降低。这些蛋白可能在TPD发生过程中发挥重要调控作用。本文结果为进一步研究泛素化在橡胶树TPD发生过程中的作用提供依据, 同时也为揭示TPD发生的分子机制提供新的思路和观点。

关键词：橡胶树; 死皮(TPD); 泛素化

收稿：2012-12-24   修定：2013-02-27

资助：中国热带农业科学院橡胶研究所人才项目(RC201204)。

Ubiquitination of Latex Protein during Tapping Panel Dryness (TPD) Process in Hevea brasiliensis Muell. Arg.

QIN Bi^1,*, LIU Chang-Ren², DU Lei³

¹Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China; ²College of Agriculture, Hainan University, Haikou 570228, China; ³College of Chemistry and Life Science, Sanxia University, Yichang, Hubei 443002, China

Corresponding author: QIN Bi; E-mail: qinbi126@163.com; Tel: 0898-23301174

Abstract:

In this study, the ubiquitination of the latex proteins from healthy and different degrees of tapping panel dryness (TPD) rubber trees (Hevea brasiliensis) was compared using Western blot. The result indicated that there was significant difference in the ubiquitination levels of latex proteins between healthy and TPD trees. In the TPD trees, the ubiquitination levels of 2 proteins were significantly increased, while 7 proteins were ob-viously decreased. These ubiquitinated proteins might play important roles in TPD. This research provides a base for further study on the roles of protein ubiquitination in TPD. Meanwhile it also provides a new perspec-tive to elucidate the molecular mechanism involved in the TPD process.

Key words: Hevea brasiliensis; tapping panel dryness (TPD); ubiquitination